DendroTEK dPGA
DendroTEK dPGA is a protease-resistant culture matrix designed to fully replace PLO/PDL/PEI and enhance cellular adherence.
Provided as a sterile, ready-to-use concentrated stock solution.
- Prevents clumping and lifting of cells - enhancing assay performance while reducing experimental risk
- Water-like viscosity, soluble in water and PBS.
- Fully synthetic, 100% protein and animal-component free
- Stock solution stable for up to 2 years
Substantially increases cell adherence, reducing detachment of cells. Completely replaces PLL/PLO/PEI as a coating in adherent cell culture.
Testimonials
Just wanted to let you know that we have been testing dPGA using our routine cortical neuron culture protocol, on non-acid washed glass imaging dishes, and it is working very well.
Dr. Richard Eva
Kings College London
DendroTEK dPGA is a game changer for the study of human iPSC-derived motor neurons for neurodegenerative diseases like ALS.
Dr. Stefano Stefani
Montreal Neurological Institute, McGill University
Upon differentiation and maturation, midbrain neurons grown on dPGA-laminin were less likely to form clumps than those on PLO-laminin, thus giving us a more uniform monolayer and consistent outcomes on our imaging-based lysosomal enzyme assays.
Dr. Yanping Zhu
Simon Fraser University
Cultures are not peeling off the chip with media changes as before. I decided that I will rely on it for future MEA recording experiments.
Dania Abdellatif
The Hebrew University of Jerusalem
We love dPGA and are using it instead of PLL for all our experiments. We find more even coating of the coverglass with dPGA than PLL.
Dr. Shernaz Bamji
University of British Columbia
ASN Neuro
2022
Dendritic Polyglycerol Amine: An Enhanced
Substrate to Support Long-Term Neural
Cell Culture (pubmed)
Journal of Neuroinflammation
2024
Fenebrutinib, a Bruton’s tyrosine kinase
inhibitor, blocks distinct human microglial
signaling pathways (pubmed)
ACS Chemical Neuroscience
2025
Characterization of Dendritic Polyglycerol Amine Layers as Coatings for Improved Neural Cell Growth Surfaces (pubmed)
dPGA supports iPSC-derived cortical neurons
Clément J-P et al. “Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture.” ASN Neuro 2022
Astrocyte-Neuron-Microglia co-culture with dPGA
Keaney J et al. “Fenebrutinib, a Bruton's tyrosine kinase inhibitor, blocks distinct human microglial signaling pathways.” Journal of Neuroinflammation 2024
dPGA as a primary coating for better Matrigel® performance
Thiry L et al. “Optimization of Long-Term Human iPSC-Derived Spinal Motor Neuron Culture Using a Dendritic Polyglycerol Amine-Based Substrate.” ASN Neuro 2022
Better MEA coverage with DendroTEK dPGA
Thiry L et al. “Optimization of Long-Term Human iPSC-Derived Spinal Motor Neuron Culture Using a Dendritic Polyglycerol Amine-Based Substrate.” ASN Neuro 2022
Reduced clumping in iPSC-derived midbrain neurons
Unpublished data shared by Yanping Zhu, Centre for High Throughput Chemical Biology, Simon Fraser University